ABSTRACT
Abstract Aneurinibacillus aneurinilyticus strain CKMV1 was isolated from rhizosphere of Valeriana jatamansi and possessed multiple plant growth promoting traits like production of phosphate solubilization (260 mg/L), nitrogen fixation (202.91 nmol ethylene mL-1 h-1), indole-3-acetic acid (IAA) (8.1 µg/mL), siderophores (61.60%), HCN (hydrogen cyanide) production and antifungal activity. We investigated the ability of isolate CKMV1 to solubilize insoluble P via mechanism of organic acid production. High-performance liquid chromatography (HPLC) study showed that isolate CKMV1 produced mainly gluconic (1.34%) and oxalic acids. However, genetic evidences for nitrogen fixation and phosphate solubilization by organic acid production have been reported first time for A. aneurinilyticus strain CKMV1. A unique combination of glucose dehydrogenase (gdh) gene and pyrroloquinoline quinone synthase (pqq) gene, a cofactor of gdh involved in phosphate solubilization has been elucidated. Nitrogenase (nif H) gene for nitrogen fixation was reported from A. aneurinilyticus. It was notable that isolate CKMV1 exhibited highest antifungal against Sclerotium rolfsii (93.58%) followed by Fusarium oxysporum (64.3%), Dematophora necatrix (52.71%), Rhizoctonia solani (91.58%), Alternaria sp. (71.08%) and Phytophthora sp. (71.37%). Remarkable increase was observed in seed germination (27.07%), shoot length (42.33%), root length (52.6%), shoot dry weight (62.01%) and root dry weight (45.7%) along with NPK (0.74, 0.36, 1.82%) content of tomato under net house condition. Isolate CKMV1 possessed traits related to plant growth promotion, therefore, could be a potential candidate for the development of biofertiliser or biocontrol agent and this is the first study to include the Aneurinibacillus as PGPR.
Subject(s)
Plant Growth Regulators/metabolism , Valerian/microbiology , Calcium Phosphates/metabolism , Solanum lycopersicum/growth & development , Bacillales/isolation & purification , Nitrogen Fixation , Soil Microbiology , Chromatography, High Pressure Liquid , Solanum lycopersicum/microbiology , Plant Roots/microbiology , Biomass , Bacillales/metabolism , Rhizosphere , Fungi/growth & development , AntibiosisABSTRACT
Background: Knowledge about the variability in the request of calcium-phosphate metabolism laboratory tests in primary care is important to design strategies to improve health system efficiency. Aim: To compare the inter-practice variability in calcium-phosphate metabolism laboratory tests requested by general practitioners from diverse regions across Spain. Material and Methods: One hundred and forty one clinical laboratories were invited to participate in an observational cross-sectional study. They informed the number of serum calcium, phosphate, parathyroid hormone and 25-hydroxyvitamin D requested by general practitioners. Appropriateness indicators were calculated as number of test requests per 1,000 inhabitants and ratio of related tests requests. The differences according to hospital setting, region and type of management were analyzed. Results: We recruited 76 laboratories (17,679,195 inhabitants). General practitioners requested 3,260,894 calcium-phosphate metabolism tests. The rate of request ranged from 2.97 per 1,000 inhabitants for 25-hydroxyvitamin D to 98.89 per 1,000 inhabitants for calcium. The rates of request for calcium, phosphate, parathyroid hormone in some areas were 30, 100 and 340 times higher than in other areas. Parathyroid hormone and 25-hydroxyvitamin D were highly requested in private management areas. There were also differences in phosphate, parathyroid hormone and 25-hydroxyvitamin D requesting between regions across Spain. Conclusions: The high variability observed is difficult to explain by differences in patient case mix between regions. Depending on the area, calcium could be under requested to detect primary hyperparathyroidism.
Objetivo: Conocer la variabilidad en la solicitud de pruebas de laboratorio en atención primaria es importante para diseñar estrategias que mejoren la eficiencia del sistema de salud. La propuesta de este estudio fue comparar la variabilidad en la solicitud de pruebas para la evaluación del metabolismo fosfocálcico por médicos de atención primaria de diversas regiones de España. Material y Método: Se invitó a participar a 141 laboratorios clínicos de diversas regiones españolas. Completaron una encuesta con el número de determinaciones de calcio, fósforo, hormona paratiroidea y 25-hidroxivitamina D solicitadas por médicos de atención primaria de sus áreas. Se calcularon las tasas en relación a la población y se construyeron indicadores de adecuación. Los resultados se compararon por características del hospital, región y tipo de gestión. Resultados: Obtuvimos los datos de 76 laboratorios (17.679.195 habitantes). Los médicos de atención primaria solicitaron 3.260.894 pruebas de metabolismo fosfocálcico. La tasa de solicitud varió de 2,97 por 1.000 habitantes de 25-hidroxivitamin D a 98,89 por 1.000 habitantes de calcio. Las tasas de calcio, fósforo, hormona paratiroidea en algunas áreas fue 30, 100 y 340 veces más alta respecto a otras. Hormona paratiroidea y 25-hidroxivitamina D fueron más solicitadas significativamente en hospitales con gestión privada. También hubo diferencias en fósforo, hormona paratiroidea y 25-hidroxivitamina D solicitas entre distintas regiones de España. Discusión: La alta variabilidad observada es difícil de explicar por las diferencias de las características de los pacientes. Dependiendo de la región podría haber una infra solicitud para la detección del hiperparatiroidismo primario.
Subject(s)
Humans , Male , Female , Primary Health Care/statistics & numerical data , Practice Patterns, Physicians' , Calcium Metabolism Disorders/diagnosis , Mass Screening/methods , Clinical Laboratory Techniques/statistics & numerical data , General Practitioners , Parathyroid Hormone/blood , Phosphates/blood , Spain , Vitamin D/analogs & derivatives , Vitamin D/blood , Calcium Phosphates/metabolism , Calcium Phosphates/blood , Cross-Sectional Studies , Hypercalcemia/diagnosis , Hyperparathyroidism/diagnosisABSTRACT
Strain P17 was a bacterial strain identified as Bacillus megaterium isolated from ground accumulating phosphate rock powder. The fermentation broth of strain P17 and the yellow-brown soil from Nanjing Agricultural University garden were collected to conduct this study. The simulation of fixed insoluble phosphorous forms after applying calcium superphosphate into yellow-brown soil was performed in pots, while available P and total P of soil were extremely positive correlative with those of groundwater. Then the dissolving effect of strain P17 on insoluble P of yellow-brown soil was studied. Results showed that Bacillus megaterium strain P17 had notable solubilizing effect on insoluble phosphates formed when too much water-soluble phosphorous fertilizer used. During 100 days after inoculation, strain P17 was dominant. Until the 120th day, compared with water addition, available P of strain P17 inoculation treated soil increased by 3 times with calcium superphosphate addition. Besides available P, pH, activity of acid and alkaline phosphatase and population of P-solubilizing microbes were detected respectively. P-solubilizing mechanism of P-solubilizing bacteria strain P17 seems to be a synergetic effect of pH decrease, organic acids, phosphatase, etc.
Subject(s)
Bacillus megaterium/metabolism , Calcium Phosphates/metabolism , Phosphorus/metabolism , Soil/chemistry , Bacillus megaterium/isolation & purification , Carboxylic Acids/metabolism , Hydrogen-Ion Concentration , Phosphoric Monoester Hydrolases/metabolism , Soil MicrobiologyABSTRACT
Alternative sources of mesenchymal stem cells (MSCs) for replacing bone marrow (BM) have been extensively investigated in the field of bone tissue engineering. The purpose of this study was to compare the osteogenic potential of canine MSCs derived from adipose tissue (AT), BM, umbilical cord blood (UCB), and Wharton's jelly (WJ) using in vitro culture techniques and in vivo orthotopic implantation assays. After canine MSCs were isolated from various tissues, the proliferation and osteogenic potential along with vascular endothelial growth factor (VEGF) production were measured and compared in vitro. For the in vivo assay, MSCs derived from each type of tissue were mixed with beta-tricalcium phosphate and implanted into segmental bone defects in dogs. Among the different types of MSCs, AT-MSCs had a higher proliferation potential and BM-MSCs produced the most VEGF. AT-MSCs and UCB-MSCs showed greater in vitro osteogenic potential compared to the other cells. Radiographic and histological analyses showed that all tested MSCs had similar osteogenic capacities, and the level of new bone formation was much higher with implants containing MSCs than cell-free implants. These results indicate that AT-MSCs, UCB-MSCs, and WJ-MSCs can potentially be used in place of BM-MSCs for clinical bone engineering procedures.
Subject(s)
Animals , Dogs , Female , Male , Adipocytes, White/cytology , Alkaline Phosphatase/metabolism , Biocompatible Materials/metabolism , Bone Diseases/therapy , Bone Marrow Cells/cytology , Calcification, Physiologic , Calcium/metabolism , Calcium Phosphates/metabolism , Cell Proliferation , Fetal Blood/cytology , Flow Cytometry , Mesenchymal Stem Cells/cytology , Osteogenesis , Polyesters/metabolism , Tissue Engineering/methods , Vascular Endothelial Growth Factor A/metabolismABSTRACT
The present study was carried out to investigate the effect of glycemic control levels in type 2 diabetic patients on the serum lipids and lipoprotein profiles and the serum levels of calcium, phosphate and some other electrolytes. The study was conducted on 81 patients with type 2 diabetes mellitus, who were attending the Outpatient Diabetic and Endocrine Clinic in King Khaled Hospital, Hail, Kingdom of Saudi Arabia. The patients were classified into two groups depending on their glycosylated hemoglobin [HbAi]; First: those with good glycemic control [GGC] who had their HbAic 7.5% [n = 43]. The control group comprised of 52 healthy subjects. All anthropometric and biochemical measurements were carried out using the standard methods. Both control and diabetic groups showed high prevalence of obesity with BMI values > 30 [32.2% of C, 34.2% of GGC and 39.5% of the PGC]. The fasting serum glucose levels in GGC and PGC groups were significantly higher than control by 77.3% and 1.1-fold, respectively. The serum total cholesterol [Tc] in the PGC group showed a significant increase [P < 0.01] by 14.4%, whereas, the GGC was not significantly different from control. Tc showed a significant positive correlation with Hb Ale [r = 0.91, P = 0.00007]. On the other hand, the serum triglyceride was significantly higher [P < 0.01] in both GGC and PGC groups. The VLDL was elevated in the GGC by 96.5% and in the PGC by 100% compared to control, whereas, the LDLc fractions in the GGC and PGC were increased by 9.1% and 16.7%, respectively, but HDL-c was not-altered. The-Serunrrrragnesium and sodium levels were not altered in any of the diabetic groups. However, calcium was significantly raised [P0.05] in the PGC compared to control, but not significantly different in GGC. Serum phosphate in the GGC and PGC exhibited significant increases [P0.001] and [P0.01], respectively. Similarly, the potassium levels were significantly higher in both groups compared to control. The type 2 diabetic patients who had better glycemic control showed favorable lipid and lipoprotein profiles with lower risk factors for atherosclerosis and had moderate calcium and phosphate levels compared to their poorly controlled counterparts
Subject(s)
Humans , Male , Female , Glycated Hemoglobin/blood , Body Mass Index , Hyperlipidemias/blood , Cholesterol/blood , Triglycerides/blood , Potassium/blood , Magnesium/blood , Calcium Phosphates/metabolismABSTRACT
La calcificación metastática ocurre frecuentemente en paciente con insuficiencia renal crónica en hemodiálisis de mantenimiento (HN). Existe dos variedades de la misma: a) calcificación visceral: pulmón, corazón, riñones, cerebro; b) calcificación no visceral: 1-arteria, ojo, piel; 2-tumoral: articular, periarticular y tejido subcutáneo. Referimos el caso de una paciente en quien se observó la infrecuente asociación de ambos tipos de calcificación. Se presentan la evolución radiológica y las tomografías computadas que se efectuaron para realizar el diagnóstico y diagnósticos diferenciales y los datos clínicos que acompañaron esas manifestaciones. Sobre la base de este caso clínico se realizan los mecanismos fisiopatogénicos de la calcificación visceral y no visceral. Se destaca la progresiva evolución del hiperparatiroidismo secundario como responsable del aumento del producto fosfocálcico en una paciente que no cumplía con la medicación instituída, lo que motivó la progresión de las calcificaciones no viscerales. Se sugiere como posible tratamiento el control de los niveles de fosfatemia a fin de disminuir el depósito de sales de calcio y sus complicaciones en el área no visceral y la necesidad de tener en cuenta a la calcificación visceral en el diagnóstico diferencial de la enfermedad cardíaca o respiratoria del paciente en HM